Journal: bioRxiv

Article Title: Effect of PEI-coated MNPs on the Regulation of Cellular Focal Adhesions and Actin Stress Fibres

doi: 10.1101/617480

Figure Lengend Snippet: (A) Fluorescence images of nucleus (purple), vinculin in blue-green (left), and F-actin in red (right). MG-63 cells were the untreated (i) control, or treated with (ii) 0.14 pg Fe/μL MNP-PEI, (iii) 0.4 pg Fe/μL MNP-PEI and (iv) 0.7 pg Fe/μL MNP-PEI. The fluorescence change from blue to green to yellow indicates increasing vinculin concentration. (B) Quantification of vinculin fluorescence intensity per area in the nucleus and cytoplasm showing significantly higher vinculin expression at high MNP-PEI doses per well (0.4 and 0.7 pg Fe/μL) N = 25 to 40 cells.

Article Snippet: Cells were incubated with a primary antibody of anti-Human/Mouse/Rat Vinculin mouse monoclonal (R&D systems; MAB68961-SP) diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam).

Techniques: Fluorescence, Concentration Assay, Expressing

Journal: Cell Reports

Article Title: SIN3A histone deacetylase action counteracts MUS81 to promote stalled fork stability

doi: 10.1016/j.celrep.2024.113778

Figure Lengend Snippet:

Article Snippet: Mouse anti Vinculin , Merk , Cat#V9264; RRID: AB_10603627.

Techniques: Recombinant, Control, Protease Inhibitor, Imaging, Reverse Transcription, Plasmid Preparation, Software, Magnetic Beads, Blocking Assay, In Situ, Western Blot, Membrane

Journal: PLoS ONE

Article Title: Paxillin Mediates Sensing of Physical Cues and Regulates Directional Cell Motility by Controlling Lamellipodia Positioning

doi: 10.1371/journal.pone.0028303

Figure Lengend Snippet: A) Primary human dermal (left) and lung (middle and right) fibroblasts align actin stress fibers (left, green; middle and right, magenta) along diagonal axes and form fibronectin (FN) fibrils (left, red) and FAs containing vinculin (middle, green) and paxillin (right, green). B) Cells stimulated with PDGF (25 ng/mL) for 30 min and stained with Alexa488-phalloidin show actin-rich protrusive structures predominantly from corner regions, defined as shown (middle). 3D reconstruction of confocal sections (right) shows that lamellipodia could fold back over cell bodies, due to a lack of adhesive substrate surrounding the adhesive islands. C) Time-lapse microscopy of GFP-paxillin in an NIH 3T3 cell stimulated with PDGF (25 ng/mL) shows large FAs that disassemble over time and paxillin-containing protrusions emanating largely from corners. Time = min after addition of PDGF. Scale bars = 10 µm.

Article Snippet: The following primary antibodies were used for immunostaining and Western blot analysis: anti-paxillin mouse monoclonal (BD Biosciences), anti-vinculin mouse monoclonal (BD Biosciences), anti-fibronectin rabbit polyclonal (Sigma), anti-myc (9E10) mouse monoclonal (Upstate), anti-GAPDH mouse monoclonal (Chemicon).

Techniques: Staining, Time-lapse Microscopy

Journal: PLoS ONE

Article Title: Paxillin Mediates Sensing of Physical Cues and Regulates Directional Cell Motility by Controlling Lamellipodia Positioning

doi: 10.1371/journal.pone.0028303

Figure Lengend Snippet: A) Myc-tagged paxillin is localized to predominantly corner-localized FAs in pax+, but not pax−/−, cells (left). Both pax+ and pax−/− cells show FAs as detected by immunostaining for vinculin (right), but pax−/− cells have more adhesive structures in side regions (red) and fewer long, fibrillar type adhesions in corner regions (blue). B) Quantification of number of FAs in corner (black bars) and side regions (white bars). n>6 cells, 400 FAs per genotype. ** p <0.005. C) Quantification of FA length in corner (black bars) and side regions (white bars). * p <0.05.

Article Snippet: The following primary antibodies were used for immunostaining and Western blot analysis: anti-paxillin mouse monoclonal (BD Biosciences), anti-vinculin mouse monoclonal (BD Biosciences), anti-fibronectin rabbit polyclonal (Sigma), anti-myc (9E10) mouse monoclonal (Upstate), anti-GAPDH mouse monoclonal (Chemicon).

Techniques: Immunostaining

Journal: Journal of Functional Foods

Article Title: Exploration of the mechanism of fermented turmeric preventing glucolipid metabolic disorder: Insights into signaling of microbiota-SCFAs-brown adipose tissue axis in mice

doi: 10.1016/j.jff.2025.106675

Figure Lengend Snippet: Fig. 2. Effects of FT on morphology and UCP1 expression of BAT. (A) Pathological sections of BAT on H&E staining. Scale bar, 100 μm. (B) BAT index (n = 6). (C) The expression of UCP1 and vinculin in BAT by WB analysis (n = 3). The expression of UCP1 and vinculin in BAT by WB analysis. Relative expression was calculated by band density. Vinculin was used as endogenous control for normalization. BAT, brown adipose tissue; UCP1, uncoupling protein 1. N, normal group; M, glucolipid metabolic disorder-model group; FT, fermented turmeric treating group. BAT index was calculated as: BAT weight (mg)/body weight (g). Values are presented as mean ± SD. * P < 0.05 significantly different from group M. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Anti-uncoupling protein 1 (UCP1), anti- hydroxyacyl-CoA dehydrogenase (HADH) and anti-vinculin antibody were purchased from Servicebio Technology Co., Ltd. (Hubei, China).

Techniques: Expressing, Staining, Control

Journal: Journal of Functional Foods

Article Title: Exploration of the mechanism of fermented turmeric preventing glucolipid metabolic disorder: Insights into signaling of microbiota-SCFAs-brown adipose tissue axis in mice

doi: 10.1016/j.jff.2025.106675

Figure Lengend Snippet: Fig. 6. The potential anti-GMD mechanism of FT based on microbiota-SCFAs-BAT axis. (A) The expression of ACSM3, HADH and vinculin in BAT by WB analysis (n = 3). (B) The relative expression levels calculated by band density (n = 3). Vinculin was used as endogenous control for normalization. (C) The comprehensive regulation of FT on microbiota-SCFAs-BAT axis. ACSM3, acyl-CoA synthetase medium chain family member 3; HADH, hydroxyacyl-CoA dehydrogenase; GMD, glucolipid metabolic disorder; SCFA, short-chain fatty acid; BAT, brown adipose tissue. N, normal group; M, glucolipid metabolic disorder-model group; FT, fermented turmeric treating group. Values are presented as mean ± SD. * P < 0.05 significantly different from group M. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Anti-uncoupling protein 1 (UCP1), anti- hydroxyacyl-CoA dehydrogenase (HADH) and anti-vinculin antibody were purchased from Servicebio Technology Co., Ltd. (Hubei, China).

Techniques: Expressing, Control